Characterization of Biofilm Adsorption – Surface Density
The two formulas listed here are pulled from the cited publications. Please refer to the original publication for a detailed description of the formula itself.
In this paper the following formula is described in detail.
Γ = 3h * (n2f - n2b)/ [(n2f + 2)(r (n2b + 2) - ν( (n2b - 1))]
Where:
- h is the height (thickness) of the bio film.
- nf is the refractive index of the film = 1.48 for protein
- nb is the refractive index of the liquid over the film (e.g. buffer)
- r is the specific refractive index increment of the film = 0.183 ml/g
- v is the partial zspecific volume of the film = 0
The role of intrinsic binding rate and transport rate in the adsorption of prothrombin, albumin, and fibrinogen to phospholipid bilayers (pages 544-554)
Jan W. Corsel, George M. Willems, Jos M. M. Kop, Peter A. Cuypers and Wim Th. Hermens
Γ = h * (nf-nb)/ dn/dc
Where:
- h is the height (thickness) of the bio film.
- dn/dc is the refractive index increment of the film components.
- nf is the refractive index of the film
- nb is the refracetive index of the liquid over the film (e.g. buffer)
Ellipsometry as a tool to study the adsorption behavior of synthetic and biopolymers at the air-water interface J. A. De Feijter, J. Benjamins, F. A. Veer
Unilever Research, Vlaardingen, The Netherlands
Biopolymers, Volume 17, Issue 7 , Pages 1759 – 1772, Published Online: 1 Feb 2004