Surface Chemistries
ICx Nomadics Recommendation for Kinetic Analysis Applications - We recommend two dimensional surface chemistry for kinetic applications as the interpretation of kinetic constants is simplified.
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Gold Only Surface
Sensor provided with gold coated glass surface only to facilitate the attachment of user-developed functional chemistry.
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COOH1
This general purpose, two dimensional coating is suitable for a wide variety of biomolecular interactions and has proven to resist non-specific binding better than any other coating. Coupling of the ligand to the surface is normally achieved via amine coupling(EDC/NHS chemistry) but alternative methods such as thiol coupling, disulfide exchange and hydrazide coupling may also be employed.
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COOH2
This modified, carboxylate surface is very similar to the COOH chemistry and may be used with the same coupling protocols. In some applications, this surface may reduce non-specific binding when compared to the standard COOH surface chemistry.
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BioCap
This is a two dimensional coating that utilizes the high affinity biotin-neutravidin interaction as the basis for immobilization of ligands. It is ideal for the capture of biotinylated reagents including peptides, oligonucleotides, proteins and sugars in a non-reversible manner.
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VesCap
The VesCap surface chemistry is a two dimensional chemistry for the immobilization of vesicles. Liposomes, unilamellar vesicles and even crude membrane preparations may be conveniently immobilized in a single step. Components embedded in the vesicles will continue to diffuse and this property makes this surface ideal for membrane bound receptor studies.
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HisCap
The HisCap surface is a two dimensional chemistry for the immobilization of His-tagged ligands.. This surface is ideal for reversible capture of polyhistidine-tagged recombinant proteins. Immobilized proteins may be removed by exposure to imidazole allowing the surface to be re-coated.
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HiCap
This is a three dimensional, polymeric surface chemistry which is ideal for amine coupling of small peptides, drugs and other small molecules that possess amine groups. Such biomolecules show negligible preconcentration and must be exposed to the surface at high concentrations... The lack of charge confers high resistance to non-specific binding. Very high binding capacities may be achieved.
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AvCap
This is a three dimensional polymeric coating utilizing biotin to capture avidin-conjugated ligands. It is a non-charged thereby limiting non-specific binding when compared to other negatively charged surfaces. It provides high capacity immobilization and is ideal for acidic proteins or where high binding capacity is required.