Specifications Unique to Pioneer
  Pioneer Pioneer ST
Gradient Injections: FastStep, Taylor Dispersion (TDi) Yes No
Determination of Diffusion Coefficient / Molecular Weight Yes No
Kinetic Analysis of Complex Binding Analytes Yes No
Kinetic curvature in single injection mode w/o affinity knowledge Yes No
Sample Configuration 96/384 well plates, deep well/PCR, Custom High Volume plates, High Volume reagent racks 96 well, deep well/PCR, Custom High Volume plates
Specifications Common to both platforms
Refractive Index Range
1.33-1.40
Short-term Noise
< 0.1 RU
Long-term Noise
< 0.3 RU
Molecular Weight Cutoff
< 100 Da
Sample Capacity
2 Sample Racks
Sample Temperature Control 4-40 °C (Max 15° below ambient)
Sample Loading
Automatic
Number of Channels
3
Flow Path
1, 1-2, 1-2-3, 3, 3-2, 3-2-1
Flow Channel Volume
< 90 nL
Channel-Channel Dead Volume
< 20 nL
Real Time Reference Curve Subtraction
Yes
Injection Volume
5-480 μL
Injection Rise and Fall Time
< 0.75 second @ 25 μL/min
Simultaneous Injections
Yes, dual sample loops
Flow Rate
5-150 μl/min
Inline Buffer Degassing
Yes
System Temperature Control
4-40 °C (Max 15° below ambient)
Variable Data Rate
1-8 Hz
Automation Capabilities
>72 hour unattended operation

Working Ranges
ka
<= 1 X 108 M-1s-1
kd
0.1 – 10-6 s-1
Concentration
~10-3 – 10-12M
KD
~10-3 – 10-12 M-1